Welcome to the Cytek Q&B cytometer validation homepage. Our web-based validation service is presently setup for making relative Q&B measurements on flow cytometers using 488 and 635nM lasers for excitation and measuring the fluorescence in the FITC, PE, PE-CY5, and APC channels (4 color, Facscan™ 4 color Facsort™ and 4 color FacsCalibur™, are example cytometers). We have also used FC-3M 7-peak Cyto-Cal Multi-Fluor calibration particles from Duke scientific for performing this test. We recommend before you get started you read our instructions for setting up your cytometer and acquiring these calibration particles here. (More Info).
Cytek's Q&B Method
Using multi-fluorescent beads with assigned MEFL a flow cytometer operator can:
  • Measures the Q or optical efficiency; this measures the relative number of photoelectrons/dye molecule for each fluorescent channel
  • Measures the B or Background; together Q and B determine the relative number of dye molecules which will be required to resolve a dim population from a negative one.
  • A hydrodynamic CV is required to complete the characterization of a channel
 
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